cfgf23 enzyme linked immunosorbent assay elisa  (R&D Systems)

Journal: Pflugers Archiv

Article Title: Controlled dietary phosphate loading in healthy young men elevates plasma phosphate and FGF23 levels

doi: 10.1007/s00424-024-03046-4

Figure Lengend Snippet: Phosphatropic hormones after 5 days of low- or high-phosphate (P i ) diet. Levels of A serum parathyroid hormone (PTH), B plasma intact fibroblast growth factor 23 (iFGF23), C plasma C-terminal FGF23 (cFGF23), D the ratio of iFGF23 (ng/l)/cFGF23 (ng/l) as well as serum E calcidiol, and F calcitriol, and G plasma soluble Klotho, and H Fetuin-A on the final day of both the 5-day low and high P i diet. One reference unit (RU)/ml corresponds to 2 ng/l cFGF23. Blue dots represent differences (Δ) between high P i diet and low P i diet expressed as mean difference ± 95% confidence interval and blue dashed line represents the zero line of the right y-axis. Data was analyzed by the paired t -test ( A – E ) or Wilcoxon test ( F–H ), n = 10, * p < 0.05

Article Snippet: Tubular maximum P i reabsorption (TmP/GFR) was calculated by using the fractional tubular reabsorption (TRP) of P i with the following equations using spot urine [ ]: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\begin{array}{l}TRP=1-\left[\left(\frac{{U}_{Pi}}{{P}_{Pi}}\right)*\left(\frac{{P}_{Cr}}{{U}_{Cr}}\right)\right]\\ TRP \le 0.86 \to TmP/GFR=TRP*{P}_{Pi}\\ TRP>0.86 \to TmP/GFR=\left(0.3*\frac{TRP}{1-\left(0.8*TRP\right)}\right)*{P}_{Pi}\end{array}$$\end{document} T R P = 1 - U Pi P Pi ∗ P Cr U Cr T R P ≤ 0.86 → T m P / G F R = T R P ∗ P Pi T R P > 0.86 → T m P / G F R = 0.3 ∗ TRP 1 - 0.8 ∗ T R P ∗ P Pi Daily fractional excretion of P i (FEP i ) was calculated by the following equation using the absolute 24-h urine P i and creatinine: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$FE{P}_{i}=\frac{{(U}_{Pi}*{P}_{Cr})}{{(P}_{Pi}*{U}_{Cr})}*100$$\end{document} F E P i = ( U Pi ∗ P Cr ) ( P Pi ∗ U Cr ) ∗ 100 Daily renal filtered P i load was calculated with the following equation: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$Renal\;P_iload/day=\left(\frac{U_{Cr}\ast U_{volume}}{P_{Cr}\ast1440}\right)\ast P_{Pi}$$\end{document} R e n a l P i l o a d / d a y = U Cr * U volume P Cr * 1440 * P Pi Plasma iFGF23, cFGF23, Fetuin-A, and urinary metanephrine were measured with the human iFGF23 and cFGF23 enzyme-linked immunosorbent assay (ELISA) (iFGF23 and cFGF23, Quidel, 60–6600 and 60–6100, respectively), Fetuin-A ELISA (R&D Systems, DFTA00, Lot P413286), and the Metanephrine Urine ELISA (Demeditec Diagnostics GmbH, DEE8400) according to manufacturers’ protocols.

Techniques: Clinical Proteomics

Journal: Pflugers Archiv

Article Title: Controlled dietary phosphate loading in healthy young men elevates plasma phosphate and FGF23 levels

doi: 10.1007/s00424-024-03046-4

Figure Lengend Snippet: Plasma/serum and urine hormones after 5 days of low- or high-phosphate (P i ) diet. Data are presented either as mean ± SD (paired t -test) or median and interquartile range (Wilcoxon test)

Article Snippet: Tubular maximum P i reabsorption (TmP/GFR) was calculated by using the fractional tubular reabsorption (TRP) of P i with the following equations using spot urine [ ]: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\begin{array}{l}TRP=1-\left[\left(\frac{{U}_{Pi}}{{P}_{Pi}}\right)*\left(\frac{{P}_{Cr}}{{U}_{Cr}}\right)\right]\\ TRP \le 0.86 \to TmP/GFR=TRP*{P}_{Pi}\\ TRP>0.86 \to TmP/GFR=\left(0.3*\frac{TRP}{1-\left(0.8*TRP\right)}\right)*{P}_{Pi}\end{array}$$\end{document} T R P = 1 - U Pi P Pi ∗ P Cr U Cr T R P ≤ 0.86 → T m P / G F R = T R P ∗ P Pi T R P > 0.86 → T m P / G F R = 0.3 ∗ TRP 1 - 0.8 ∗ T R P ∗ P Pi Daily fractional excretion of P i (FEP i ) was calculated by the following equation using the absolute 24-h urine P i and creatinine: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$FE{P}_{i}=\frac{{(U}_{Pi}*{P}_{Cr})}{{(P}_{Pi}*{U}_{Cr})}*100$$\end{document} F E P i = ( U Pi ∗ P Cr ) ( P Pi ∗ U Cr ) ∗ 100 Daily renal filtered P i load was calculated with the following equation: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$Renal\;P_iload/day=\left(\frac{U_{Cr}\ast U_{volume}}{P_{Cr}\ast1440}\right)\ast P_{Pi}$$\end{document} R e n a l P i l o a d / d a y = U Cr * U volume P Cr * 1440 * P Pi Plasma iFGF23, cFGF23, Fetuin-A, and urinary metanephrine were measured with the human iFGF23 and cFGF23 enzyme-linked immunosorbent assay (ELISA) (iFGF23 and cFGF23, Quidel, 60–6600 and 60–6100, respectively), Fetuin-A ELISA (R&D Systems, DFTA00, Lot P413286), and the Metanephrine Urine ELISA (Demeditec Diagnostics GmbH, DEE8400) according to manufacturers’ protocols.

Techniques: Clinical Proteomics

Journal: Pflugers Archiv

Article Title: Controlled dietary phosphate loading in healthy young men elevates plasma phosphate and FGF23 levels

doi: 10.1007/s00424-024-03046-4

Figure Lengend Snippet: Associations between phosphatropic hormones and tubular maximal phosphate reabsorption. Non-linear regression analysis of plasma intact fibroblast growth factor 23 (iFGF23) ( A ), C-terminal FGF23 (cFGF23) ( B ), and serum parathyroid hormone (PTH) ( C ) with tubular maximum phosphate (P i ) reabsorption (TmP/GFR) and of serum calcitriol ( D ), plasma cFGF23 ( E ), and serum PTH ( F ) with plasma iFGF23. A straight black line was fitted and ROUT method for outlier removal was applied. Black and red dots represent participants on low or high P i diet, respectively. Blue dots represent identified outliers. The dashed line represents 95% confidence interval. The slope was analyzed by the extra sum-of-squares F test. Significance level p < 0.05

Article Snippet: Tubular maximum P i reabsorption (TmP/GFR) was calculated by using the fractional tubular reabsorption (TRP) of P i with the following equations using spot urine [ ]: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\begin{array}{l}TRP=1-\left[\left(\frac{{U}_{Pi}}{{P}_{Pi}}\right)*\left(\frac{{P}_{Cr}}{{U}_{Cr}}\right)\right]\\ TRP \le 0.86 \to TmP/GFR=TRP*{P}_{Pi}\\ TRP>0.86 \to TmP/GFR=\left(0.3*\frac{TRP}{1-\left(0.8*TRP\right)}\right)*{P}_{Pi}\end{array}$$\end{document} T R P = 1 - U Pi P Pi ∗ P Cr U Cr T R P ≤ 0.86 → T m P / G F R = T R P ∗ P Pi T R P > 0.86 → T m P / G F R = 0.3 ∗ TRP 1 - 0.8 ∗ T R P ∗ P Pi Daily fractional excretion of P i (FEP i ) was calculated by the following equation using the absolute 24-h urine P i and creatinine: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$FE{P}_{i}=\frac{{(U}_{Pi}*{P}_{Cr})}{{(P}_{Pi}*{U}_{Cr})}*100$$\end{document} F E P i = ( U Pi ∗ P Cr ) ( P Pi ∗ U Cr ) ∗ 100 Daily renal filtered P i load was calculated with the following equation: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$Renal\;P_iload/day=\left(\frac{U_{Cr}\ast U_{volume}}{P_{Cr}\ast1440}\right)\ast P_{Pi}$$\end{document} R e n a l P i l o a d / d a y = U Cr * U volume P Cr * 1440 * P Pi Plasma iFGF23, cFGF23, Fetuin-A, and urinary metanephrine were measured with the human iFGF23 and cFGF23 enzyme-linked immunosorbent assay (ELISA) (iFGF23 and cFGF23, Quidel, 60–6600 and 60–6100, respectively), Fetuin-A ELISA (R&D Systems, DFTA00, Lot P413286), and the Metanephrine Urine ELISA (Demeditec Diagnostics GmbH, DEE8400) according to manufacturers’ protocols.

Techniques: Clinical Proteomics

Journal: Pflugers Archiv

Article Title: Controlled dietary phosphate loading in healthy young men elevates plasma phosphate and FGF23 levels

doi: 10.1007/s00424-024-03046-4

Figure Lengend Snippet: Phosphatropic hormones after 5 days of low- or high-phosphate (P i ) diet. Levels of A serum parathyroid hormone (PTH), B plasma intact fibroblast growth factor 23 (iFGF23), C plasma C-terminal FGF23 (cFGF23), D the ratio of iFGF23 (ng/l)/cFGF23 (ng/l) as well as serum E calcidiol, and F calcitriol, and G plasma soluble Klotho, and H Fetuin-A on the final day of both the 5-day low and high P i diet. One reference unit (RU)/ml corresponds to 2 ng/l cFGF23. Blue dots represent differences (Δ) between high P i diet and low P i diet expressed as mean difference ± 95% confidence interval and blue dashed line represents the zero line of the right y-axis. Data was analyzed by the paired t -test ( A – E ) or Wilcoxon test ( F–H ), n = 10, * p < 0.05

Article Snippet: Tubular maximum P i reabsorption (TmP/GFR) was calculated by using the fractional tubular reabsorption (TRP) of P i with the following equations using spot urine [ ]: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\begin{array}{l}TRP=1-\left[\left(\frac{{U}_{Pi}}{{P}_{Pi}}\right)*\left(\frac{{P}_{Cr}}{{U}_{Cr}}\right)\right]\\ TRP \le 0.86 \to TmP/GFR=TRP*{P}_{Pi}\\ TRP>0.86 \to TmP/GFR=\left(0.3*\frac{TRP}{1-\left(0.8*TRP\right)}\right)*{P}_{Pi}\end{array}$$\end{document} T R P = 1 - U Pi P Pi ∗ P Cr U Cr T R P ≤ 0.86 → T m P / G F R = T R P ∗ P Pi T R P > 0.86 → T m P / G F R = 0.3 ∗ TRP 1 - 0.8 ∗ T R P ∗ P Pi Daily fractional excretion of P i (FEP i ) was calculated by the following equation using the absolute 24-h urine P i and creatinine: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$FE{P}_{i}=\frac{{(U}_{Pi}*{P}_{Cr})}{{(P}_{Pi}*{U}_{Cr})}*100$$\end{document} F E P i = ( U Pi ∗ P Cr ) ( P Pi ∗ U Cr ) ∗ 100 Daily renal filtered P i load was calculated with the following equation: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$Renal\;P_iload/day=\left(\frac{U_{Cr}\ast U_{volume}}{P_{Cr}\ast1440}\right)\ast P_{Pi}$$\end{document} R e n a l P i l o a d / d a y = U Cr * U volume P Cr * 1440 * P Pi Plasma iFGF23, cFGF23, Fetuin-A, and urinary metanephrine were measured with the human iFGF23 and cFGF23 enzyme-linked immunosorbent assay (ELISA) (iFGF23 and cFGF23, Quidel, 60–6600 and 60–6100, respectively), Fetuin-A ELISA (R&D Systems, DFTA00, Lot P413286), and the Metanephrine Urine ELISA (Demeditec Diagnostics GmbH, DEE8400) according to manufacturers’ protocols.

Techniques:

Journal: Pflugers Archiv

Article Title: Controlled dietary phosphate loading in healthy young men elevates plasma phosphate and FGF23 levels

doi: 10.1007/s00424-024-03046-4

Figure Lengend Snippet: Plasma/serum and urine hormones after 5 days of low- or high-phosphate (P i ) diet. Data are presented either as mean ± SD (paired t -test) or median and interquartile range (Wilcoxon test)

Article Snippet: Tubular maximum P i reabsorption (TmP/GFR) was calculated by using the fractional tubular reabsorption (TRP) of P i with the following equations using spot urine [ ]: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\begin{array}{l}TRP=1-\left[\left(\frac{{U}_{Pi}}{{P}_{Pi}}\right)*\left(\frac{{P}_{Cr}}{{U}_{Cr}}\right)\right]\\ TRP \le 0.86 \to TmP/GFR=TRP*{P}_{Pi}\\ TRP>0.86 \to TmP/GFR=\left(0.3*\frac{TRP}{1-\left(0.8*TRP\right)}\right)*{P}_{Pi}\end{array}$$\end{document} T R P = 1 - U Pi P Pi ∗ P Cr U Cr T R P ≤ 0.86 → T m P / G F R = T R P ∗ P Pi T R P > 0.86 → T m P / G F R = 0.3 ∗ TRP 1 - 0.8 ∗ T R P ∗ P Pi Daily fractional excretion of P i (FEP i ) was calculated by the following equation using the absolute 24-h urine P i and creatinine: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$FE{P}_{i}=\frac{{(U}_{Pi}*{P}_{Cr})}{{(P}_{Pi}*{U}_{Cr})}*100$$\end{document} F E P i = ( U Pi ∗ P Cr ) ( P Pi ∗ U Cr ) ∗ 100 Daily renal filtered P i load was calculated with the following equation: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$Renal\;P_iload/day=\left(\frac{U_{Cr}\ast U_{volume}}{P_{Cr}\ast1440}\right)\ast P_{Pi}$$\end{document} R e n a l P i l o a d / d a y = U Cr * U volume P Cr * 1440 * P Pi Plasma iFGF23, cFGF23, Fetuin-A, and urinary metanephrine were measured with the human iFGF23 and cFGF23 enzyme-linked immunosorbent assay (ELISA) (iFGF23 and cFGF23, Quidel, 60–6600 and 60–6100, respectively), Fetuin-A ELISA (R&D Systems, DFTA00, Lot P413286), and the Metanephrine Urine ELISA (Demeditec Diagnostics GmbH, DEE8400) according to manufacturers’ protocols.

Techniques:

Journal: Pflugers Archiv

Article Title: Controlled dietary phosphate loading in healthy young men elevates plasma phosphate and FGF23 levels

doi: 10.1007/s00424-024-03046-4

Figure Lengend Snippet: Associations between phosphatropic hormones and tubular maximal phosphate reabsorption. Non-linear regression analysis of plasma intact fibroblast growth factor 23 (iFGF23) ( A ), C-terminal FGF23 (cFGF23) ( B ), and serum parathyroid hormone (PTH) ( C ) with tubular maximum phosphate (P i ) reabsorption (TmP/GFR) and of serum calcitriol ( D ), plasma cFGF23 ( E ), and serum PTH ( F ) with plasma iFGF23. A straight black line was fitted and ROUT method for outlier removal was applied. Black and red dots represent participants on low or high P i diet, respectively. Blue dots represent identified outliers. The dashed line represents 95% confidence interval. The slope was analyzed by the extra sum-of-squares F test. Significance level p < 0.05

Article Snippet: Tubular maximum P i reabsorption (TmP/GFR) was calculated by using the fractional tubular reabsorption (TRP) of P i with the following equations using spot urine [ ]: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\begin{array}{l}TRP=1-\left[\left(\frac{{U}_{Pi}}{{P}_{Pi}}\right)*\left(\frac{{P}_{Cr}}{{U}_{Cr}}\right)\right]\\ TRP \le 0.86 \to TmP/GFR=TRP*{P}_{Pi}\\ TRP>0.86 \to TmP/GFR=\left(0.3*\frac{TRP}{1-\left(0.8*TRP\right)}\right)*{P}_{Pi}\end{array}$$\end{document} T R P = 1 - U Pi P Pi ∗ P Cr U Cr T R P ≤ 0.86 → T m P / G F R = T R P ∗ P Pi T R P > 0.86 → T m P / G F R = 0.3 ∗ TRP 1 - 0.8 ∗ T R P ∗ P Pi Daily fractional excretion of P i (FEP i ) was calculated by the following equation using the absolute 24-h urine P i and creatinine: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$FE{P}_{i}=\frac{{(U}_{Pi}*{P}_{Cr})}{{(P}_{Pi}*{U}_{Cr})}*100$$\end{document} F E P i = ( U Pi ∗ P Cr ) ( P Pi ∗ U Cr ) ∗ 100 Daily renal filtered P i load was calculated with the following equation: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$Renal\;P_iload/day=\left(\frac{U_{Cr}\ast U_{volume}}{P_{Cr}\ast1440}\right)\ast P_{Pi}$$\end{document} R e n a l P i l o a d / d a y = U Cr * U volume P Cr * 1440 * P Pi Plasma iFGF23, cFGF23, Fetuin-A, and urinary metanephrine were measured with the human iFGF23 and cFGF23 enzyme-linked immunosorbent assay (ELISA) (iFGF23 and cFGF23, Quidel, 60–6600 and 60–6100, respectively), Fetuin-A ELISA (R&D Systems, DFTA00, Lot P413286), and the Metanephrine Urine ELISA (Demeditec Diagnostics GmbH, DEE8400) according to manufacturers’ protocols.

Techniques:

Journal: Endocrinology

Article Title: Acute Parathyroid Hormone Injection Increases C-Terminal but Not Intact Fibroblast Growth Factor 23 Levels

doi: 10.1210/en.2016-1451

Figure Lengend Snippet: Acute effects of a single PTH injection in WT mice. Baseline cFGF23 levels in different experiments ranged between 218 ± 19 and 345 ± 34 pg/mL, and peak cFGF23 levels ranged between 578 ± 114 and 1244 ± 110 pg/mL; therefore, changes in cFGF23 (and iFGF23) levels are reported as percentage of change from baseline. (a) Plasma phosphate levels 2, 6, and 24 hours after PTH or vehicle injection. (b) Plasma cFGF23 levels, expressed as percentage over preinjection baseline, at 2, 4, 6, and 24 hours after PTH injection. (c) Plasma iFGF23 levels, expressed as percentage over preinjection baseline, at 2, 4, 6, and 24 hours after PTH injection. n = 5 or more mice per group. (d) Ratio of cleaved/total FGF23 was calculated as follows: (value measured by cFGF23 ELISA − value measured by iFGF23 ELISA)/value measured by cFGF23 ELISA. n = 7–8 per group. *P < 0.05.

Article Snippet: FGF23 was measured using the rodent-specific cFGF23 and iFGF23 enzyme-linked immunosorbent assay (ELISA) kits (Immutopics, San Clemente, CA).

Techniques: Injection, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

Journal: Endocrinology

Article Title: Acute Parathyroid Hormone Injection Increases C-Terminal but Not Intact Fibroblast Growth Factor 23 Levels

doi: 10.1210/en.2016-1451

Figure Lengend Snippet: Dose-response effects of cFGF23 and iFGF23 after PTH injection. PTH doses of 12.5 and 50 nmol/kg were injected, and (a) peak cFGF23 levels (at 2 hours) and (b) nadir iFGF23 levels (at 6 hours) are shown. Absolute values are shown from an individual experiment. n = 5 or more mice per group. Bars show significant response by analysis of variance.

Article Snippet: FGF23 was measured using the rodent-specific cFGF23 and iFGF23 enzyme-linked immunosorbent assay (ELISA) kits (Immutopics, San Clemente, CA).

Techniques: Injection

Journal: Endocrinology

Article Title: Acute Parathyroid Hormone Injection Increases C-Terminal but Not Intact Fibroblast Growth Factor 23 Levels

doi: 10.1210/en.2016-1451

Figure Lengend Snippet: Effects of PTH injection in ADHR mice or in animals pretreated with the furin inhibitor 537076 (at concentration 7.5 μg/g) or PBS vehicle. (a) cFGF23 and (b) iFGF23 changes after PTH injection (50 nmol/kg), or vehicle (10 mm citric acid, 150 mm NaCl, 0.05% Tween 80, pH 5.0) in ADHR mutant mice. n = 6–8 per group. Baseline cFGF23 levels in ADHR mutant mice in different experiments ranged between 190 ± 13 and 281 ± 19 pg/mL, and peak cFGF23 levels ranged between 485 ± 78 and 1242 ± 239 pg/mL, whereas baseline iFGF23 levels in ADHR mutant mice in different experiments ranged between 262 ± 23 and 345±18 pg/mL, and peak iFGF23 levels ranged between 450 ± 25 and 975 ± 149 pg/mL. Changes in cFGF23 and iFGF23 levels are reported as percentage of change from baseline. (c) cFGF23 and (d) iFGF23 levels in WT mice all injected with PTH; furin refers to mice pretreated with furin inhibitor, and vehicle refers to mice pretreated with vehicle as described. n > 4 mice per group. *P < 0.05. Hashed line shows 100% of baseline.

Article Snippet: FGF23 was measured using the rodent-specific cFGF23 and iFGF23 enzyme-linked immunosorbent assay (ELISA) kits (Immutopics, San Clemente, CA).

Techniques: Injection, Concentration Assay, Mutagenesis